MSIPepPublications › 10.1002/pmic.200800624
Detergent addition to tryptic digests and ion mobility separation prior to MS/MS improves peptide yield and protein identification for in situ proteomic investigation of frozen and formalin‐fixed paraffin‐embedded adenocarcinoma tissue sections
- Authors
- Djidja M, Francese S, Loadman PM, Sutton CW, Scriven P, Claude E, Snel MF, Franck J, Salzet M, Clench MR
- Journal
- PROTEOMICS (2009)
- Mass tolerance
- 75 ppm – m/z reported to 0.1 Da; rounding alone gives errors up to about ±60 ppm.
- DOI
- 10.1002/pmic.200800624
Experiments
- Homo sapiens (human) · Tumour (site not stated) · Tumour
Observations (37)
| Peptide ▲ | Protein | Measured m/z | Calc. m/z | ppm | Species · tissue | Source | Status |
|---|---|---|---|---|---|---|---|
AGLQFPVGR |
Histone H2A.Z P0C0S5 |
944.5000 | 944.5312 | -33.1 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
AM[Oxidation]GIM[Oxidation]NSFVNDIFER |
Histone H2B type 1-B P33778 |
1775.8000 | 1775.8091 | -5.1 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
AMGIMNSFVNDIFER |
Histone H2B type 1-B P33778 |
1743.8000 | 1743.8193 | -11.1 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
DISSSLNSLADSNAREFEEESK |
Metastasis-associated protein MTA2 O94776 |
2428.2000 | 2428.1110 | +36.6 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
DLYANTVLSGGTTMYPGIADR |
Actin, cytoplasmic 1 P60709 |
2215.1000 | 2215.0700 | +13.6 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
DNIQGITKPAIR |
Histone H4 P62805 |
1325.7000 | 1325.7536 | -40.4 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
[Oxidation]?FMTILRR |
2-oxoglutarate dehydrogenase-like, mitochondrial Q9ULD0 |
952.5000 | 952.5397 | -41.7 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
FNALQYLR |
Lumican P51884 |
1024.6000 | 1024.5574 | +41.5 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
GPVVSAPLGAAR |
Calmodulin-lysine N-methyltransferase Q7Z624 |
1094.6000 | 1094.6317 | -28.9 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
HLQLAIR |
Histone H2A.Z P0C0S5 |
850.5000 | 850.5257 | -30.3 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
HLQLAIRNDEELNKLLGK |
Histone H2A type 1 P0C0S8 |
2104.2000 | 2104.1873 | +6.0 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
HPDYSVVLLLR |
Albumin P02768 |
1311.7000 | 1311.7419 | -32.0 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
KLPFQR |
Histone H3.3C Q6NXT2 |
788.5000 | 788.4777 | +28.2 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
K[Dimethyl]SAPATGGVK[Dimethyl]KPHR |
Histone H3.1 P68431 |
1489.9000 | 1489.8962 | +2.6 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
LAHYNKR |
Histone H2B type 1-B P33778 |
901.5000 | 901.5003 | -0.3 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
LLVVYPWTQR |
Hemoglobin subunit beta P68871 |
1274.7000 | 1274.7256 | -20.1 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
LVGMPAK |
Stress-70 protein, mitochondrial P38646 |
715.4000 | 715.4171 | -23.9 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
[Oxidation]?MFLSFPTTK |
Hemoglobin subunit alpha P69905 |
1087.6000 | 1087.5492 | +46.7 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
MSGKVTKPKEEK |
Isoform 3 of Fibroblast growth factor 13 Q92913-3 |
1361.7000 | 1361.7457 | -33.6 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
[Oxidation]?NHKGGVPPQR |
Small ribosomal subunit protein mS40 Q9Y676 |
1105.6000 | 1105.5861 | +12.6 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
[Oxidation]?RCPNHELSR |
Tumor protein 63 Q9H3D4 |
1127.6000 | 1127.5375 | +55.5 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
RDLDDLKK |
Sodium/potassium-transporting ATPase subunit alpha Q53ES0 |
1002.5000 | 1002.5578 | -57.7 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
RHPDYSVVLLLR |
Albumin P02768 |
1467.8000 | 1467.8431 | -29.3 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
RHPDYSVVLLLR |
Albumin P02768 |
1467.8000 | 1467.8431 | -29.3 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
[Oxidation]?RPLSGGHVTR |
UL-16 binding protein 5 Q6H3X3 |
1095.6000 | 1095.6018 | -1.6 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
RPPSAFFLFCSEYRPK |
High mobility group protein B1 P09429 |
1944.9000 | 1944.9789 | -40.6 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
RSGGHAPR |
Estrogen receptor beta Q92731 |
837.4000 | 837.4438 | -52.3 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
SGKVTKPK |
Isoform 3 of Fibroblast growth factor 13 Q92913-3 |
844.5000 | 844.5251 | -29.7 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
STELLIR |
Histone H3.3C Q6NXT2 |
831.0000 | 831.4934 | -593.4 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Needs review |
SYELPDGQVITIGNER |
Actin, cytoplasmic 1 P60709 |
1790.9000 | 1790.8919 | +4.5 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
TVTAMDVVYALKR |
Histone H4 P62805 |
1466.8000 | 1466.8036 | -2.4 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
TVTAMDVVYALKR |
Histone H4 P62805 |
1466.8000 | 1466.8036 | -2.4 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
VAPEEHPVLLTEAPLNPK |
Actin, cytoplasmic 1 P60709 |
1954.1000 | 1954.0644 | +18.2 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
VGAGAPVYLAAVLEYLTAEILELAGNAAR |
Histone H2A type 1 P0C0S8 |
2915.6000 | 2915.5877 | +4.2 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
VGAHAGEYGAEALER |
Hemoglobin subunit alpha P69905 |
1529.7000 | 1529.7343 | -22.4 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
VGAHAGEYGAEALER |
Hemoglobin subunit alpha P69905 |
1529.7000 | 1529.7343 | -22.4 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |
VTKPKEEK |
Isoform 3 of Fibroblast growth factor 13 Q92913-3 |
958.6000 | 958.5568 | +45.1 | Human · Tumour (site not stated) Tumour |
10.1002/pmic.200800624 | Approved |